Übersicht Eigenschaften der DNA Exonucleasen
Removal of ssDNA and oligonucleotides.
||3´?5´ exonuclease, it requires the presence of magnesium and a free 3´-hydroxyl terminus; wide range of buffer conditions
||15 min, 80°C|
Preparation of ssDNA templates for sequencing.
||5´?3´ exonuclease that digests one strand of dsDNA from 5´-phosphorylated blunt or recessed ends. It has low activity on 5´-hydroxyl ends and is not active on nicked DNA.
||dNMPs and ssDNA on the opposite strand. Partial digestion produces dsDNA having 3´ extensions of ssDNA.
||10 min, 75°C|