DNA Exonucleasen
 
Übersicht Eigenschaften der DNA Exonucleasen
 
 
Enzyme
Applications
Substrate Activity Products Heat Inactivation
Exonuclease I,
E. coli
(280430)

Removal of ssDNA and oligonucleotides.
ssDNA 3´?5´ exonuclease, it requires the presence of magnesium and a free 3´-hydroxyl terminus; wide range of buffer conditions  dNMPs 15 min, 80°C
Lambda Exonuclease
(280440)


Preparation of ssDNA templates for sequencing.
dsDNA 5´?3´ exonuclease that digests one strand of dsDNA from 5´-phosphorylated blunt or recessed ends. It has low activity on 5´-hydroxyl ends and is not active on nicked DNA. dNMPs and ssDNA on the opposite strand. Partial digestion produces dsDNA having 3´ extensions of ssDNA. 10 min, 75°C
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

              
Exonuclease I, 20000 U/ml
30000 Units
Art.-Nr.: 280430 (X8010L)



€ 364,00
 
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