DNA Exonucleasen
Übersicht Eigenschaften der DNA Exonucleasen
Enzyme
Applications |
Substrate |
Activity |
Products |
Heat Inactivation |
Exonuclease I,
E. coli
(280430)
Removal of ssDNA and oligonucleotides. |
ssDNA |
3´?5´ exonuclease, it requires the presence of magnesium and a free 3´-hydroxyl terminus; wide range of buffer conditions |
dNMPs |
15 min, 80°C |
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Lambda Exonuclease
(280440)
Preparation of ssDNA templates for sequencing. |
dsDNA |
5´?3´ exonuclease that digests one strand of dsDNA from 5´-phosphorylated blunt or recessed ends. It has low activity on 5´-hydroxyl ends and is not active on nicked DNA. |
dNMPs and ssDNA on the opposite strand. Partial digestion produces dsDNA having 3´ extensions of ssDNA. |
10 min, 75°C |