DNA und RNA Ligasen
 
Übersicht Eigenschaften der DNA und RNA Ligasen
 
 
 
 
 
 
 
 
 
 
 
 
Enzyme Ligation
Temp
Cofactor Ligation Template Type of Ends Ligated Primary Application Heat Inactivation
  Required to Ligate Blunt Cohesive    
T3 DNA Ligase
(280210)
20°C ATP No Yes Yes Tolerates High Ionic Strength  
T4 DNA Ligase
(280220)
4°C-25°C ATP No Yes Yes Cloning 15 min, 65°C
T4 DNA Ligase (Rapid)
(280225)
25°C ATP No Yes Yes Rapid Ligation  
T7 DNA Ligase
(280250)
25°C ATP No Yes Yes 1000 fold higher activity on sticky ends than blunt ends  
Taq DNA Ligase
(280260)
45°-65°C NAD Yes; DNA only No Yes Ligase Chain reaction No; half-life 30 min, 95°C
E. coli DNA Ligase
(280200)
5°C-25°C NAD Yes; DNA only Weak activity Yes Make long cDNA 20 min, 65°C
Fast-Link™ DNA Ligase
(133600)
20°C ATP No Yes Yes Rapid ligation 10 min, 65°C
Thermostable RNA Ligase
(280230)
25°C-60°C ATP No Ligates ssRNA N/A Ligate ssRNA to ssRNA or ssDNA 5 min, 100°C
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

              
T4 DNA Ligase, 120000 U/ml
150000 Units
Art.-Nr.: 280220 (L6030-LC-L)



€ 364,00
 
T4 DNA Ligase (Rapid), 600000 U/ml
240000 Units
Art.-Nr.: 280225 (L6030-HC-L)



€ 515,00
 
Fast-Link DNA Ligations Kit
Lucigen
100 Reaktionen
Art.-Nr.: 133600 (LK6201H)



€ 250,00
 
Fast-Link DNA Ligations Kit
Lucigen
50 Reaktionen
Art.-Nr.: 133605 (LK0750H)



€ 153,00
 
E. coli DNA Ligase, 10000 U/ml
2500 Units
Art.-Nr.: 280200 (L6090L)



€ 364,00
 
T3 DNA Ligase, 3000000 U/ml
900000 Units
Art.-Nr.: 280210 (L6010L)



€ 364,00
 
T7 DNA Ligase, 3000000 U/ml
900000 Units
Art.-Nr.: 280250 (L6020L)



€ 364,00
 
Taq DNA Ligase, 40000 U/ml
20000 Units
Art.-Nr.: 280260 (L6060L)



€ 364,00
 
T4 RNA Ligase 1, 20000 U/ml
10000 Units
Art.-Nr.: 280230 (L6050L)



€ 364,00
 
T4 RNA Ligase 2, 30000 U/ml
4500 Units
Art.-Nr.: 280240 (L6080L)



€ 364,00
 
T4 RNA Ligase 2 Truncated, 5000 U/ml
500 Units
Art.-Nr.: 280245 (L6070L)



€ 364,00
 
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