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DNA und RNA Ligasen
Übersicht Eigenschaften der DNA und RNA
Ligasen
| Enzyme |
Ligation
Temp |
Cofactor |
Ligation
Template |
Type of Ends
Ligated |
Primary
Application |
Heat
Inactivation |
| |
Required to
Ligate |
Blunt |
Cohesive |
|
|
T3 DNA
Ligase
(280210) |
20°C |
ATP |
No |
Yes |
Yes |
Tolerates High Ionic Strength |
|
T4 DNA
Ligase
(280220) |
4°C-25°C |
ATP |
No |
Yes |
Yes |
Cloning |
15 min, 65°C |
T4 DNA Ligase
(Rapid)
(280225) |
25°C |
ATP |
No |
Yes |
Yes |
Rapid Ligation |
|
T7 DNA
Ligase
(280250) |
25°C |
ATP |
No |
Yes |
Yes |
1000 fold higher activity on sticky ends
than blunt ends |
|
Taq DNA
Ligase
(280260) |
45°-65°C |
NAD |
Yes; DNA only |
No |
Yes |
Ligase Chain reaction |
No; half-life 30 min, 95°C |
E.
coli DNA Ligase
(280200) |
5°C-25°C |
NAD |
Yes; DNA only |
Weak activity |
Yes |
Make long cDNA |
20 min, 65°C |
Fast-Link™ DNA
Ligase
(133600) |
20°C |
ATP |
No |
Yes |
Yes |
Rapid ligation |
10 min, 65°C |
Thermostable RNA
Ligase
(280230) |
25°C-60°C |
ATP |
No |
Ligates ssRNA |
N/A |
Ligate ssRNA to ssRNA or ssDNA |
5 min, 100°C |
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