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TransforMax EC100 elektrokompetente E. coli

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10 x 100 µl

  • Artikel-Nr.: 190055
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  • Hersteller-Nr.: EC10010
334,80 € *

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Applications Routine cloning of DNA up to 200 kb. The highly versatile... mehr
Produktinformationen "TransforMax EC100 elektrokompetente E. coli"
Applications
  • Routine cloning of DNA up to 200 kb.
The highly versatile TransforMax™ EC100™ E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table 1).
 
 
 
Benefits
  • High transformation efficiency with clones of all sizes, including BAC clones (Table 1).
  • lacZΔM15 for blue/white screening of recombinants.
  • Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.
  • Endonuclease minus (endA1) to ensure high yields of DNA.
  • Recombination minus (recA1) for greater stability of large cloned inserts.
 

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG

TransforMax EC100 Electrocompetent E. coli

  • Transformation efficiency of >1 x 1010 cfu/µg of pUC19.

TransforMax EC100 Chemically Competent E. coli

  • Transformation efficiency of >5 x 108 cfu/µg of pUC19.

 

DNA TransforMax™ EC100™ Chemically Competent E. coli TransforMax™ EC100™ Electrocompetent E. coli
pUC19 1.4 x 108 1.4 x 1010
8.1-kb Clone 1.3 x 107 Not tested
13.1-kb Clone 4.3 x 106 1.3 x 109
23.1-kb Clone 9.2 x 105 3.0 x 108
145-kb BAC Clone Not tested 7 x 107
13.1-kb clone directly from a ligation reaction 2.2 x 105 2.1 x 107

 

 

Table 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with a variety of DNAs. Transformations were performed using 50 µl of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µl aliquot from a standard 10-µl ligation reaction. Results shown are in cfu/µg of DNA and are the average transformation efficiencies obtained from several trials.

 

 

Produkttyp: Kompetente Zellen
Verpackung: 10 x 100 µl
Weiterführende Links zu "TransforMax EC100 elektrokompetente E. coli"
Applications Routine cloning of DNA up to 200 kb. The highly versatile... mehr