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Nukleasen

Enzyme Substrate Activity Products Applications Heat Inactivation
Exonuclease III,
 E. coli
(162025)
Exonuclease III,
 E. coli
(280435)		 dsDNA 3´→5´ exonuclease that digests one strand of duplex DNA from the 3´ end of a nick or a blunt or 3´-recessed end; not active on thionucleotides. Exo III also has 3´-DNA phosphatase and apurinic DNA endonuclease activities. dNMPs and ssDNA on the opposite strand. Partial digestion produces dsDNA having 5´ extensions of ssDNA. Used with S1 Nuclease or Mung Bean Nuclease to make nested deletions. Preparation of ssDNA templates for sequencing. Site-directed mutagenesis. Preparation of labeled strand-specific probes. 20 min, 70°C
RNase-Free DNase I (bovine pancreas, 170500,171000) dsDNA and ssDNA Activated by divalent cations. In presence of Mg2+, it cleaves each DNA strand randomly and independently, preferentially adjacent to pyrimidines. In presence of Mn2+, it cleaves both strands simultaneously, generating fragments with blunt ends of 1-2-base overhangs Oligos and dNMPs with 5´ phosphate and 3´-OH Removing DNA from RNA preps. Random nicking of dsDNA. DNase footprinting 20 min, 75°C
Baseline-ZERO™ Dnase (170005) dsDNA and ssDNA Digests dsDNA or ssDNA down to mononucleotides Mononucleotides Removing DNA from RNA preps 20 min, 75°C
Plasmid-Safe™ ATP-Dependent Dnase (161010,161100) linear ssDNA and dsDNA Selectively digests linear DNA. No activity on nicked or closed-circular DNA. dNMPs Remove chromosomal DNA fragments from plasmid, fosmid, and BAC preparations. NT
Exonuclease I, E. coli (162200)

 Exonuclease I, E. coli (280430)		 ssDNA 3´→5´ exonuclease,  it requires the presence of magnesium and a free 3´-hydroxyl terminus; wide range of buffer conditions  dNMPs Removal of ssDNA and oligonucleotides. 15 min, 80°C

Exonuclease VII
(162225)

 ssDNA Exonuclease that digests in both 5´→3´ and 3´→5´ directions. dNMPs Removal of primers and single-stranded oligos. 10 min, 95°C
Rec J Exonuclease (162337) ssDNA 5´→3´ exonuclease that digests ssDNA with a 5´ phosphate or a 5´OH. dNMPs Removal of primers and ssDNA from dsDNA. 20 min, 65°C

Terminator™ 5´-Phosphate-Dependent Exonuclease
(162370)

 ssDNA or ssRNA 5´→3´ exonuclease that digests ssDNA or ssRNA with 5´-monophosphorylated ends, but not with 5´-hydroxyl, 5´-triphosphorylated, or 5´-capped ends dNMPs or NMPs Removal of 5´-monophosphorylated DNA or primers or oligos. Enrichment of ssDNA or ssRNA molecules lacking 5´-monophosphate groups 10 min, 65°C
OmniCleave™ Endonuclease
(129350)		 ssDNA, dsDNA, or RNA Endonuclease that efficiently digests DNA and RNA di-, tri-, and tetra-nucleotides Removal of DNA and RNA from protein preps. Removal of host DNA from phage preps No
RNase H, E. coli
(280510)		 RNA in RNA:DNA hybrid Cleaves RNA in RNA:DNA hybrid without affecting unhybridized RNA or DNA. Oligoribonucleotides with 5´ phosphate and 3´ OH Elimination of RNA prior to second-strand cDNA synthesis. Removal of poly(A) tails from mRNA hybridized to oligo(DT). 10 min, 65°C
Hybridase™ Thermostable RNase H
(108211)		 RNA in RNA:DNA hybrid Cleaves RNA in RNA:DNA hybrid without affecting unhybridized RNA or DNA. Oligoribonucleotides with 5´ phosphate and 3´ OH High-stringency hybrid selection. No
RNase A
(160630)		 ssRNA Cleaves ssRNA 3´ of pyrimidine residues. Oligoribonucleotides with 3´-cytidine or 3´-uridine residues Removal of RNA from DNA preps. RNase protection assays. RNA mapping and structure studies. NT
RNase I, E. coli
(173010)		 ssRNA Cleaves ssRNA between all dinucleotide pairs. NMPs with 5´-OH and 2´,3´-cyclic monophosphate Removal of RNA from DNA preps. RNase protection assays. Mismatch detection of single basepairs in RNA:RNA or RNA:DNA hybrids. 15 min, 70°C

RNase R
(172010)

 linear RNA-not circular or lariat Magnesium-dependent 3´→5´ exoribonuclease    Alternative splicing and gene expression studies ,Intron cDNA production, Intronic screening of cDNA libraries  
Enzyme Substrate Activity Products Applications Heat Inactivation Exonuclease III,  E. coli (162025) Exonuclease III,  E. coli (... mehr erfahren »
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Nukleasen
Enzyme Substrate Activity Products Applications Heat Inactivation
Exonuclease III,
 E. coli
(162025)
Exonuclease III,
 E. coli
(280435)		 dsDNA 3´→5´ exonuclease that digests one strand of duplex DNA from the 3´ end of a nick or a blunt or 3´-recessed end; not active on thionucleotides. Exo III also has 3´-DNA phosphatase and apurinic DNA endonuclease activities. dNMPs and ssDNA on the opposite strand. Partial digestion produces dsDNA having 5´ extensions of ssDNA. Used with S1 Nuclease or Mung Bean Nuclease to make nested deletions. Preparation of ssDNA templates for sequencing. Site-directed mutagenesis. Preparation of labeled strand-specific probes. 20 min, 70°C
RNase-Free DNase I (bovine pancreas, 170500,171000) dsDNA and ssDNA Activated by divalent cations. In presence of Mg2+, it cleaves each DNA strand randomly and independently, preferentially adjacent to pyrimidines. In presence of Mn2+, it cleaves both strands simultaneously, generating fragments with blunt ends of 1-2-base overhangs Oligos and dNMPs with 5´ phosphate and 3´-OH Removing DNA from RNA preps. Random nicking of dsDNA. DNase footprinting 20 min, 75°C
Baseline-ZERO™ Dnase (170005) dsDNA and ssDNA Digests dsDNA or ssDNA down to mononucleotides Mononucleotides Removing DNA from RNA preps 20 min, 75°C
Plasmid-Safe™ ATP-Dependent Dnase (161010,161100) linear ssDNA and dsDNA Selectively digests linear DNA. No activity on nicked or closed-circular DNA. dNMPs Remove chromosomal DNA fragments from plasmid, fosmid, and BAC preparations. NT
Exonuclease I, E. coli (162200)

 Exonuclease I, E. coli (280430)		 ssDNA 3´→5´ exonuclease,  it requires the presence of magnesium and a free 3´-hydroxyl terminus; wide range of buffer conditions  dNMPs Removal of ssDNA and oligonucleotides. 15 min, 80°C

Exonuclease VII
(162225)

 ssDNA Exonuclease that digests in both 5´→3´ and 3´→5´ directions. dNMPs Removal of primers and single-stranded oligos. 10 min, 95°C
Rec J Exonuclease (162337) ssDNA 5´→3´ exonuclease that digests ssDNA with a 5´ phosphate or a 5´OH. dNMPs Removal of primers and ssDNA from dsDNA. 20 min, 65°C

Terminator™ 5´-Phosphate-Dependent Exonuclease
(162370)

 ssDNA or ssRNA 5´→3´ exonuclease that digests ssDNA or ssRNA with 5´-monophosphorylated ends, but not with 5´-hydroxyl, 5´-triphosphorylated, or 5´-capped ends dNMPs or NMPs Removal of 5´-monophosphorylated DNA or primers or oligos. Enrichment of ssDNA or ssRNA molecules lacking 5´-monophosphate groups 10 min, 65°C
OmniCleave™ Endonuclease
(129350)		 ssDNA, dsDNA, or RNA Endonuclease that efficiently digests DNA and RNA di-, tri-, and tetra-nucleotides Removal of DNA and RNA from protein preps. Removal of host DNA from phage preps No
RNase H, E. coli
(280510)		 RNA in RNA:DNA hybrid Cleaves RNA in RNA:DNA hybrid without affecting unhybridized RNA or DNA. Oligoribonucleotides with 5´ phosphate and 3´ OH Elimination of RNA prior to second-strand cDNA synthesis. Removal of poly(A) tails from mRNA hybridized to oligo(DT). 10 min, 65°C
Hybridase™ Thermostable RNase H
(108211)		 RNA in RNA:DNA hybrid Cleaves RNA in RNA:DNA hybrid without affecting unhybridized RNA or DNA. Oligoribonucleotides with 5´ phosphate and 3´ OH High-stringency hybrid selection. No
RNase A
(160630)		 ssRNA Cleaves ssRNA 3´ of pyrimidine residues. Oligoribonucleotides with 3´-cytidine or 3´-uridine residues Removal of RNA from DNA preps. RNase protection assays. RNA mapping and structure studies. NT
RNase I, E. coli
(173010)		 ssRNA Cleaves ssRNA between all dinucleotide pairs. NMPs with 5´-OH and 2´,3´-cyclic monophosphate Removal of RNA from DNA preps. RNase protection assays. Mismatch detection of single basepairs in RNA:RNA or RNA:DNA hybrids. 15 min, 70°C

RNase R
(172010)

 linear RNA-not circular or lariat Magnesium-dependent 3´→5´ exoribonuclease    Alternative splicing and gene expression studies ,Intron cDNA production, Intronic screening of cDNA libraries  
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