Phosphatasen und Kinasen

Enzyme

Substrate

Activity

Products

Applications

CapClip Pyrophosphatase (187005)

Eukaryotic mRNA with cap structure or RNA with 5´triphophorylated end

CapClip Pyrophosphatase hydrolyzes the phosphoric acid anhydride bonds in the triphosphate bridge of the cap structure found in most eukaryotic mRNA

5´-monophosphorylated terminus on the RNA molecule

Preparation of templates for 5´ RACE, Ligation of oligoribonucleotides to TAP-treated cellular RNA for construction of full-length cDNA libraries

RNA 5´ Polyphosphatase (136120)

RNA with 5´triphophorylated end

Sequential removal of the γ and β phosphates from 5´-triphosphorylated RNA5´ , pppN—OH 3´ → 5´ pN—OH 3´ + 2 P_i

5´-monophosphorylated terminus on the RNA molecule

Conversion of 5´-triphosphorylated RNA to 5´-monophosphorylated RNA for use in 5´-RNA ligation-tagging methods using T4 RNA Ligase, Preparation of substrate RNA molecules for subsequent degradation using Lucigen`s Terminator™ 5´-Phosphate-Dependent Exonuclease

T4 Polynucleotide Kinase (135030)

ssDNA, dsDNA, RNA

Catalyzes the transfer of the γ-phosphate from ATP to the 5´ hydroxyl of ssDNA and dsDNA, RNA, and nucleoside 3´ monophosphates, removes the 3´ phosphate from 3´-phosphoryl polynucleotides, deoxyribonucleoside 3´ monophosphates, and deoxyribonucleoside-3´,5´-diphosphates to form a 3´-hydroxyl group.

Phosphorylation of oligonucleotide linkers and other DNA or RNA molecules prior to ligation