Verschiedene Polymerasen für Ihre PCR
Sie interessieren sich für thermostabile Polymerasen für die PCR? Diese finden Sie hier
Bezeichnung | 5′–>3′ Exo. | 3′–>5′ Exo. | Nick Transl. | Strand Displ. | Thermal Stability | Resulting ends | Applications |
No | Yes | No | Yes | No | Blunt | DNA blunting by fill-in of 5’ overhang; Second strand cDNA synthesis | |
Exo-Minus Klenow DNA Polymerase 10 U/µl (131223, 280315) | No | No | No | Yes | No | 3 'A | A-tailing for NGS, DNA Labeling |
DNA Polymerase l, 10000 U/ml (280300) | Yes | Yes | Yes | No | No | Blunt | Second strand cDNA synthesis, Nick-translation, DNA Labeling |
Mako DNA Polymerase, 30000 U/ml | No | No | No | No | No | ||
phi29 DNA Polymerase, 100000 U/ml (280340) | No | Yes | No | Yes | No | Blunt | Whole Genome Amplification |
T4 DNA Polymerase, 3000 U/ml | No | Yes | No | No | No | Blunt | Blunting, DNA end-repair by fill-in of 5’ overhang |
MasterAmp Tth DNA Polymerase + Puffer, 5 U/µl (103025) | Yes | No | Yes | PCR-RTPCR |