TransforMax EC100 elektrokompetente E. coli
Artikelinformationen "TransforMax EC100 elektrokompetente E. coli"
Applications
- Routine cloning of DNA up to 200 kb.
The highly versatile TransforMax™ EC100™ E. coli
competent cells are ideal for most cloning applications. The cells provide
very high transformation efficiency when tested against a wide range of
supercoiled DNAs as well as DNA directly from a ligation reaction (Table
1).
Benefits
- High transformation efficiency with clones of all sizes, including BAC clones (Table 1).
- lacZΔM15 for blue/white screening of recombinants.
- Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.
- Endonuclease minus (endA1) to ensure high yields of DNA.
- Recombination minus (recA1) for greater
stability of large cloned inserts.
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG
TransforMax EC100 Electrocompetent E. coli
- Transformation efficiency of >1 x 1010 cfu/µg of pUC19.
TransforMax EC100 Chemically Competent E. coli
- Transformation efficiency of >5 x 108 cfu/µg of pUC19.
| DNA | TransforMax™ EC100™ Chemically Competent E. coli | TransforMax™ EC100™ Electrocompetent E. coli |
| pUC19 | 1.4 x 108 | 1.4 x 1010 |
| 8.1-kb Clone | 1.3 x 107 | Not tested |
| 13.1-kb Clone | 4.3 x 106 | 1.3 x 109 |
| 23.1-kb Clone | 9.2 x 105 | 3.0 x 108 |
| 145-kb BAC Clone | Not tested | 7 x 107 |
| 13.1-kb clone directly from a ligation reaction | 2.2 x 105 | 2.1 x 107 |
Table 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with a variety of DNAs. Transformations were performed using 50 µl of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µl aliquot from a standard 10-µl ligation reaction. Results shown are in cfu/µg of DNA and are the average transformation efficiencies obtained from several trials.
| Produkttyp: | Kompetente Zellen |
|---|---|
| Verpackung: | 10 x 100 µl |
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